Improved membrane assay using focused sample application.

摘要

Methods and kits are described for performing immunoassays. The kit includes a reaction cell (80) having a microporous membrane (92) and an absorbent (96) capable of drawing liquid sample through the membrane. An applicator (84) is provided for applying a small volume of the liquid sample to the membrane by contacting a port (102) of the applicator to the membrane and allowing the sample to flow therethrough by capillary action. Analyte in the sample is immobilized, typically by immunoadsorption, and the immobilized analyte may then be visualized using conventional signal producing systems, such as color, fluorescence, and luminescent systems.