DIHYDROFOLIC ACID REDUCTASE-SOMASTATIN FUSED PROTEIN

摘要

PURPOSE:To enable the separation and purification of a dihydrofolic acid reductase-somatostatin fused protein produced by E.coli transformed with plasmid pTPGIF2, by purifying the protein using a specific chromatography. CONSTITUTION:Escherichia coli containing plasmid pTPGIF2 (FERM BP-1577) is cultured and the obtained bacterial cells are disintegrated and centrifuged. The cell-free extraction liquid of the supernatant is subjected to ion-exchange column, methotrexate-bonded affinity column chromatography and anion- exchange column chromatography using dihydrofolic acid reductase activity as a criterion. The objective dihydrofolic acid reductase-somatostatin fused protein shown in the figure can be produced by this process in purified state.