LEUKOCYTE DIFFERENTIATION COMPOSITION AND METHOD

摘要

A composition and method are disclosed for differentiating white blood cells. The composition comprises at least one surfactant and at least one dilute acid which together selectively strip the cytoplasm from certain classes of white blood cells and not others. More particularly, the composition causes lysis of lymphocytes, monocytes, eosinophils and neutrophils, but not basophils. Thus, basophils are differentiated from other PMN sub-classes by their appearance as intact cells. The composition and method totally avoid the need for dye preparation and the vagaries of staining techniques and can be used either manually or with instrumentation.