| 摘要 |
A peptide is sequenced from its carboxyl terminal by coupling with an isothiocyanate having a chemical formula R(x)Si(NCS)y where x=0-3 and y=1-4. R may be alkyl or aryl. The coupling reaction is preferably conducted at a temperature range of 50 DEG -90 DEG C. for a period to approximately sixty (60) minutes or less. A weak acid anhydride, preferably a carboxylic acid anhydride, or a weak acid chloride, preferably a carboxylic acid chloride, may be mixed with the isothiocyanate. An organic base catalyst selected from a group consisting of tertiary amines including aromatic amines such as pyridine may also be utilized.
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