| 摘要 |
Process for producing anti-freeze peptides (AFP), by expressing in a host organism, preferably a yeast, a gene encoding AFP controlled by a strong promoter, e.g. an inducible GAL7 or a constitutive GAPDH promoter of (Saccharomyces cerevisiae). The AFP gene can be preceded by a signal sequence for secretion of the protein, e.g. the invertase signal sequence or the pre-sequence of the (alpha)-mating factor of (S. cerevisiae). The non-AFP part in the fused gene can be part of the (alpha)-galactosidase gene of (Cyamopsis tetragonoloba). The gene construct can comprise a processing site that can be used to split off at least one AFP from the remainder of the protein. The AFP gene can be present as a tadem-repeat of AFP genes. The invention also relates to AFP produced as described above and to the use of such AFP for inhibiting ice crystal growth, e.g. in ice cream and other food products, or other biological materials that can be frozen. And further to a yeast having improved freeze tolerance, containing and/or surrounded by AFP produced as described above, which can be used in producing frozen dough or other compositions that can be frozen. |
