METHOD AND APPARATUS FOR FIXATION OF BIOPOLYMERS TO A SOLID SUPPORT BY CENTRIFUGATION

摘要

The invention provides an apparatus and method for affixing charged biopolymers to porous supports. The invention permits standardization between separate samples and relieves the inefficiency and contamination attendant to conventional vacuum methods. The apparatus (10) includes a sample tube (12) having a plurality of sample chambers (14) with tubular exit ports (22), for receiving multiple samples, and a transverse porous support (44) at the base of exit ports (22). The support (44) is held in tight contact with the sample by a removable head (30) with exit ports (22) which effects a liquid, tight seal surrounding each exit port so as to reduce cross mixing of sample. The sample tube may be inserted into an elutant reservoir such as a centrifuge tube and centrifuged so as to effect flow of the sample through the support (44) resulting in the fixation of the biopolymer onto the support.