发明名称 |
PRODUCTION OF ANIMAL LYSOZYME C VIA SECRETION FROM PICHIA PASTORIS AND COMPOSITION THEREFOR |
摘要 |
The present invention provides a method of producing at high levels an animal lysozyme c by culturing Pichia pastoris cells, which have a gene capable of expressing the pre-form of the animal lysozyme c in such cells, under conditions such that the gene is transcribed. Mature animal lysozyme c is secreted at a high level from such cells into the culture medium, when the cells are cultured under conditions such that the animal pre-lysozyme c gene is transcribed in them. Also provided by the invention are DNAs capable of transforming Pichia pastoris to express animal pre-lysozyme c's, cultures of P. pastoris cells transformed with such DNAs, bovine pre-lysozyme c2, the signal peptide of bovine pre-lysozyme c2, and DNAs encoding those polypeptides. |
申请公布号 |
AU2825489(A) |
申请公布日期 |
1989.06.01 |
申请号 |
AU19890028254 |
申请日期 |
1989.11.02 |
申请人 |
THE SALK INSTITUTE BIOTECHNOLOGY/INDUSTRIAL ASSOCIATES, INC. |
发明人 |
MARY ELLEN DIGAN;MICHAEL MILLER HARPOLD;STEPHEN VERNON LAIR;GREGORY PATRICK THILL;ROBERT STEVEN SIEGEL;STEVEN BRADLEY ELLIS;MARK EDWARD WILLIAMS |
分类号 |
A61K38/46;A61P31/04;C12N1/19;C12N9/36;C12N15/09;C12N15/81;C12R1/84 |
主分类号 |
A61K38/46 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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