| 摘要 |
In prepn. of a crosslinked biopolymer based on collagen by alkaline salt treatment of the eye sclera of animals, homogenisation of the tissue obtd. with an aq. soln. of organic acid until a collagen soln. forms, and then extn. of low-mol. impurities, (a) the collagen soln. freed from impurities is satd. with N2O, (b) the soln. is brought to a concn. of at most 80 wt.% and (c) the soln. is irradiated with ionising radiation at a dose of 0.5-15 kGy until a crosslinked biopolymer forms. - Pref. before irradiation, the collagen soln. is partly degassed by centrifuging for 10-30 mins. at 3-40 x 10 power(3) rpm. In prodn. of an implant, (i) the collagen soln. freed from impurities is brought to a concn. of 0.2-1.3 wt.%, (ii) the soln. is satd. with N2O, (iii) a mould is charged with the soln. and irradiated with ionising radiation at a dose of 0.5-15 kGy, (iv) the implant blank, of crosslinked biopolymer based on collagen, is dialysed for 24-48 hrs. in water, (v) the blank is dried to a moisture content of 30-50 wt.% in the crosslinked biopolymer, and (vi) the blank is given the shape and size of the implant, to ensure complete sealing of wounds to the cornea of corium in eye injuries. Before being charged into the mould, the soln. may be partly degassed by successive freezing and thawing at 20-25 deg.C and centrifuging for 10-60 mins. at 1-2 x10 power(3) rpm. The implant is shaped so that it is a cylinder with tapered end, with a length of 3-5 mm and a dia. of the base of 0.5-4.0 mm. |
