MONOCLONAL ANTIBODY RECOGNIZING 2-3 BOND

摘要

PURPOSE:To produce a monoclonal antibody recognizing alpha2 3 bond by making a hybridoma producing the monoclonal antibody with a specificity to a specific lipid having alpha2 3 bond. CONSTITUTION:B-cells (lymphocytes) obtained by immunizing an animal using a glycolipid containing sialic acid containing N-acetylneuramin hearing alpha2 3 bond is subjected to cell fusion with myeloma cells to produce a hybridoma. The hybridoma produces a monoclonal antibody having specificity to a sialic acid-containing glycolipid containing N-acetylneuramin bearing alpha2 3 bond as an epitope. The hybridoma is allowed to produce the antibody by the cloning and the limiting dilution analysis.