Method for cloning in lactic acid bacteria.

摘要

A plasmid-borne gene from a first lactic acid bacterium is cloned in a second lactic acid bacterium by means of a cloning vector that contains a gene for a phenotypic marker and that is devoid of an origin of replication for the second bacterium. The plasmid from the first lactic acid bacterium is combined with the cloning vector to produce a recombinant plasmid that is easily selectable in the second lactic acid bacterium and that contains both the gene to be cloned and an origin of replication for the second lactic acid bacterium. The recombinant plasmid or plasmid-borne functional gene segment so identified can be inserted into a lactic acid bacterial host by conjugation or transformation to provide an improved starter culture bacterial strain.