摘要 |
A multizone element and method are designed for use for the determination of an analyte in whole blood. In particular, this element and method is useful for the quantitative determination of peroxide-generating analytes, such as glucose or cholesterol, in whole blood. The multizone element has a support having thereon, in order and in fluid contact, a registration zone and a reagent/spreading zone but excludes a whole blood cell filter zone. The reagent/spreading zone has a void volume and average pore size effective to accomodate whole blood, and contains an interactive composition necessary for the analysis. Such composition is capable of providing, upon interaction with the analyte, a dye which can be spectrophotometrically detected at a wavelength greater than or equal to 600 nm. |