Immobilized luciferase for the quantitative determination of ATP

摘要

Luciferase is immobilized on a membrane prepared from an absorbent albuminoid such as procine skin gelatin. The membrane may be formed by spreading a solution of porcine skin gelatin on a support and after incubation and drying removing the resultant membrane from the support. Preferably, the membrane is crosslinked with a solution of glutaraldehyde, and luciferase is immobilized by saturating the membrane with luciferase in the presence of dithiothreitol. The immobilized luciferase is particularly suitable for the quantitative determination of adenosine triphosphoric acid (ATP).