| 摘要 |
<p>Nucleic acid probes are provided which are chemically tagged with moieties which chelate the trivalent lanthanides Eu?+3, Tb+3 and Sm+3¿. Also provided are methods of making said probes and methods of using the probes in hybridization assays. The probes of the invention are detected, preferably by time-resolved fluorometry, by means of the intense, long-lived fluorescence of Eu?+3, Tb+3 and Sm+3¿, particularly in chelates with aromatic trifluoromethyl β-diketones, such as 2-naphthoyltrifluoroacetone, and synergistic bases, such as tri-n-octylphosphine oxide, when such chelates are in micelles, such as those formed in water with non-ionic detergents such as Triton X-100.</p> |
