MANIFESTATION VECTOR HAVING DNA CODING NON-A NON-B HEPATITIS SPECIFIC ANTIGEN, TRANSFORMANT AND PRODUCTION OF SAID ANTIGEN

摘要

PURPOSE:To produce a non-A non-B hepatitis specific antigen, by introducing a DNA-containing DNA fragment coding a non-A non-B hepatitis specific antigen in a cloning site existing at the downstream side of a promoter, thereby forming a manifestation vector. CONSTITUTION:A DNA fragment containing a DNA coding a non-A non-B hepatitis specific antigen is introduced into a cloning site existing at the downstream side of a promoter of manifestation vector. The obtained manifestation vector containing the DNA fragment is introduced into a host to effect the transformation of the host and the resultant transformant is cultured. The non-A non-B hepatitis specific antigen produced and accumulated in the cultured product is separated therefrom A large amount of non-A non-B hepatitis specific antigen protein can be produced by this process at a low cost.