发明名称 |
ANALYTE ASSAY UTILIZING MOLECULAR BRIDGES, MODIFIERS AND SIGNAL GENERATORS |
摘要 |
A method of detecting in a sample an analyte (A) having a molecularly recognizable portion thereon, which comprises: providing (B) a molecular bridging entity having thereon: (i) a portion capable of recognizing the molecularly recognizable portion on the analyte; and (ii) a portion comprising a polynucleotide sequence; and (C) a signalling entity having thereon: (i) a polynucleotide portion capable of annealing to the polynucleotide portion of the bridging entity, thereby to form a stable polynucleotide hybrid, and (ii) a signal generating portion; forming a complex comprising: (1) the analyte (A) complexed through its molecularly recognizable portion to (2) the recognizing portion of the entity (B); the entity (B) being complexed through the polynucleotide portion thereon to (3) the polynucleotide portion of the signalling entity; and detecting a signal by means of the signal generating portion present in the complex. |
申请公布号 |
AU583787(B2) |
申请公布日期 |
1989.05.11 |
申请号 |
AU19840027675 |
申请日期 |
1984.05.04 |
申请人 |
ENZO BIOCHEM, INC. |
发明人 |
ROBERT G. PERGOLIZZI;JANNIS G. STAVRIANOPOULOS;ELAZAR RABBANI;DEAN L. ENGELHARDT;STAN KLINE |
分类号 |
G01N33/48;C07H21/04;C12Q1/68;G01N33/50;G01N33/53;G01N33/58;(IPC1-7):C07G17/00;C07H21/02 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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