摘要 |
PCT No. PCT/JP85/00715 Sec. 371 Date Oct. 10, 1986 Sec. 102(e) Date Oct. 10, 1986 PCT Filed Dec. 26, 1985 PCT Pub. No. WO86/04067 PCT Pub. Date Jul. 17, 1986.A method of purifying a polypeptide having a physiological activity such as one having interferon activities from a culture mixture of a microorganism obtained by a recombinant DNA technique and capable of producing the polypeptide is disclosed. The method comprises subjecting the cultured cells to extraction and purification in the presence of a salt of zinc or copper and polyethyleneimine thereby inhibiting decomposition and denaturation of the polypeptide. The extracted polypeptide can be further purified by column chromatographies using a column containing an anion exchange resin, column containing a cation exchange resin and column containing a gel filtration resin.
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申请人 |
HIGASHI, NAOKI;SUGIMOTO, SHUNJIRO;TSUJIMOTO, MASAFUMI;SHIRASAWA, HOUNAI;OKADA, TSUTOMU;YAMAMOTO, KAZUMORI;NAGABHUSHAN, TATTANAHALLI L.;TROTTA, PAUL P. |
发明人 |
HIGASHI, NAOKI;SUGIMOTO, SHUNJIRO;TSUJIMOTO, MASAFUMI;SHIRASAWA, HOUNAI;OKADA, TSUTOMU;YAMAMOTO, KAZUMORI;NAGABHUSHAN, TATTANAHALLI L.;TROTTA, PAUL P. |