发明名称 NUCLEIC ACID HYBRIDIZATION ASSAY EMPLOYING IMMOBILIZED RNA PROBES
摘要 <p>A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA-RNA or RNA-RNA hybrids with the particular polynucleotide sequence to be determined. Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution.</p>
申请公布号 CA1253777(A) 申请公布日期 1989.05.09
申请号 CA19850481294 申请日期 1985.05.10
申请人 MILES LABORATORIES, INC. 发明人 CARRICO, ROBERT J.
分类号 C07H21/00;C07K16/00;C07K16/44;C12N5/10;C12N15/02;C12P21/08;C12Q1/68;C12R1/91;G01N33/53;G01N33/543;G01N33/577;(IPC1-7):G01N33/53 主分类号 C07H21/00
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