发明名称 |
System for metering nutrient media to cell culture containers and method |
摘要 |
A system for metering nutrient media to cell culture containers includes a media flow conduit which defines at one end means for substantially aseptic communication with the source of the media. In a preferred embodiment, sterile connectors may be used for essentially sterile conditions. A branch conduit extends from the flow conduit at a location spaced from the ends. The branch conduit communicates with means for receiving a predetermined-volume aliquot of media through the media flow conduit from said one end. The flow conduit also defines a portion downstream from the branch conduit, which communicates with at least one cell culture container, each container being a flexible bag. At least part of the flexible bag is made of a material capable of providing at least 2 times the oxygen diffusion and at least 2 times the carbon dioxide diffusion between the bag interior and exterior, compared with an identically-sized, sealed bag having polyethylene walls 0.013 inch thick.
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申请公布号 |
US4829002(A) |
申请公布日期 |
1989.05.09 |
申请号 |
US19860862344 |
申请日期 |
1986.05.12 |
申请人 |
BAXTER INTERNATIONAL INC. |
发明人 |
PATTILLO, MARTHA C.;BACEHOWSKI, DAVID V.;BILSTAD, ARNOLD C.;CULLIS, HERBERT M.;DENNEHEY, T. MICHAEL;YANG, JAMES W.;BROWN, WILLIAM C. |
分类号 |
C12M3/00;C12M1/00;C12M1/26;C12N5/00;C12N5/10 |
主分类号 |
C12M3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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