| 摘要 |
<p>Fowlpox virus (FPV) promoter DNA for use in expressing a foreign gene inserted in an FPV vector by homologous recombination, which comprises the promoter of any of the following FPV genes: (1) the FP4b gene which encodes a protein of about 657 amino acids in a sequence beginning Met Glu Ser Asp Ser Asn Ile Ala Ile Glu Glu Val Lys Tyr Pro Asn Ile Leu Leu Glu; (2) the BamHI fragment ORF8 gene encoding a protein of about 116 amino acids in a sequence beginning Met Glu Glu Gly Lys Pro Arg Arg Ser Ser Ala Val Leu Trp Met Leu Ile Pro Cys Gly; (3) The BamHI fragment ORF5 gene encoding a protein of about 105 amino acids in a sequence beginning Met Ile Ile Arg Arg Asn Asn Lys Ala Leu Gly Ser Val Met Ser Asp Phe Ile Lys Thr; (4) The BamHI fragment ORF10 gene encoding a protein of about 280 amino acids in a sequence beginning Met Lys Phe Lys Glu Val Arg Asn Thr Ile Lys Lys Met Asn Ile Thr Asp Ile Lys Ile; and (5) the gene of which the coding stand hybridises strongly to FPV RNA and is at least partly located within an approximately 790 bp DNA sequence, containing near its 5'-end the sequence: (5') TGTCATCATA TCCACCTATA AATGTAATAT and near its 3'-end the sequence: AAGAATAGTC TAAATTACCT AACATAGAAC ATCAT (3').</p> |
