| 摘要 |
The concentration of molecular oxygen in a sample is determined by exciting the oxygen molecules in the sample from the electronic ground state to the excited 1δg state (excited singlet state) by measuring a 1270 nm luminescence characteristic for the excited oxygen molecules (singlet oxygen), and by correlating the measured luminescence characteristic with the concentration of molecular oxygen in the sample. The 1270 nm luminescence characteristic is normally the 1270 nm luminescence intensity. The oxygen molecules are preferably excited by being exposed to diffusion contact with a sensitizer such as porphyrin or a porphyrin- related compound, for example a transition metal porphyrin complex. The sensitizer is brought into the oxygen-excited electronic state by absorption of electromagnetic radiation such as light, the oxygen- excited electronic state being, if appropriate, adapted to the measuring system used by means of a quencher such as a substituted polyene. The sensitizer can be present in an organic solvent or a polymer such as polyvinylchloride. The oxygen-containing sample is preferably a sample of biological origin such as a blood sample.
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