VISUALIZATION METHOD FOR THE DIRECT OR INDIRECT DETECTION OF THE REACTION BETWEEN A SPECIFIC BINDING AGENT AND THE CORRESPONDING ACCEPTOR SUBSTANCE IN BLOT OVERLAY ASSAYS

摘要

JAB 438 VISUALIZATION METHOD FOR THE DIRECT OR INDIRECT DETECTION OF THE REACTION BETWEEN A SPECIFIC BINDING AGENT AND THE CORRESPONDING ACCEPTOR SUBSTANCE IN BLOT OVERLAY ASSAYS A method for detecting and/or determining an agglomerate formed by the reaction between a specific binding agent and the corresponding acceptor substance according to the general methodology of blot overlay assays by using colloidal metal particles labelled components which may be visualized as a coloured signal at the surface of the blotting medium characteristic for the colloidal metal particles used or quantitatively determined following art-known spectophotometric procedures such as densitometry.