METHOD FOR PRODUCTION OF ANDROSTA-1,4-DIENE-3,17-DIONE FROM PLANT AND ANIMAL DERIVED STEROLS

摘要

FIELD: biotechnology, microbiology, chemistry, pharmaceutical industry. ^ SUBSTANCE: invention relates to microbial production of androsta-1,4-diene-3,17-dione (ADD). Claimed method is based on ability of specific Mycobacterium neoaurum bacteria strain to selectively eliminate side chain of abovementioned sterols and to accumulate ADD in presence of transformation product sorbent. As sorbents synthetic polymer resins obtained by copolymerization of divinylbenzene and ethylstyrene are used. Sorbents are added in broth in end of lag-phase. ADD is formed with yield of 66-76 % depending on side chain structure of transformed substrates. ^ EFFECT: improved method of ADD production. ^ 5 ex