METHOD OF OBTAINING POLYPEPTIDES IN CELL-FREE TRANSLATION SYSTEM.

摘要

Polypeptides are obtained in a cell-free translation system on ribosomes which contains, as substrates, ATP, GTP and amino acids with the resulting translation products containing the desired product, AMP, GDP, pyrophosphate and inorganic phosphate. In the course of the translation process, as the substrates are consumed and the products are obtained, the products of translation including AMP, GDP, pyrophosphate, inorganic phosphate and the desired product are extracted from the system, while simultaneously introducing in the system substrates in the form of amino acids, ATP and GTP for the purpose of maintaining their initial concentration.