| 摘要 |
Specific determination of the serum fructosamine (I) content of blood (or derived) samples comprises (1) eliminating non-specific non-specific reducing and/or turbidity-producing components; (2) reacting, at pH 10-12, with an appropriate colour-reagent and (3) measuring the resulting colour change. The new feature is that non-specific reducing components are eliminated by treating at about neutral pH with one or more enzymatic oxidising agents (A), opt. together with peroxidase and/or catalase,plus one or more SH-gp. blocking agents (B). Also new are reagents for this process. (A) are ascorbate oxidase, bilirubin oxidase and/or uricase, and (B) are iodoacetamide (pref.), an iodoacetate, N-ethylmaleimide and p-hydroxymercuribenzoate. |
