| 发明名称 |
Method of gene amplification for enhancing protein production in cultured animal cells. |
| 摘要 |
<p>Genes are effectively amplified in animal cells by transfecting the cells with the gene to be amplified and a hygromycin B-resistance gene and culturing the cells in a medium containing hygromycin B. The product encoded by the amplified gene is obtained in high yield.</p> |
| 申请公布号 |
EP0313009(A2) |
申请公布日期 |
1989.04.26 |
| 申请号 |
EP19880117380 |
申请日期 |
1988.10.19 |
| 申请人 |
HOECHST JAPAN LIMITED |
发明人 |
MIZUSHIMA, YUKU;NAKAUCHI, HIROMITSU;OKUMURA, KO;WATANABE, HIROSHI;SUGAWARA, ISAMU |
| 分类号 |
C07K14/705;C12N15/09;C12N5/10;C12N15/67;C12N15/85;C12P21/00;C12R1/91 |
主分类号 |
C07K14/705 |
| 代理机构 |
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代理人 |
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| 主权项 |
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| 地址 |
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