| 摘要 |
Crude interferon was purified by adsorption, ion-exchange and gel chromatography. Thus, crude interferon was applied to Nu-Gel AC column equilibrated with 20mM phosphate buffer (pH 7.4, 0.15M NaCl), washed with 20mM phosphate buffer (pH 7.4, 0.15M NaCl) and 0.1M Tris buffer (pH 8.0, 0.25M NH4Cl), and eluted with 0.1M Tris buffer [pH 8.0, 0.5M (CH3)4NCl . The eluted interferon fraction was dialyzed against 1 liter phosphate buffer (20mM, pH 6.0) over 4times, applied to Nu-Gel P-DE column equilibrated with phosphate buffer (20mM, pH 6.0), washed with 20mM phosphate buffer (pH 6.0), washed with 20mM phosphate buffer (pH 6.0) and 20mM phosphate buffer (pH 6.0, 0.15M NaCl), and eluted with 20mM phosphate buffer (pH 6.0, 1M NaCl).
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