发明名称 |
Polyacrylamide gel particles having hapten moieties bound thereto as immunoassay reagent |
摘要 |
An immobilized hapten reagent for use in a specific binding assay for the determination of a hapten or binding analog thereof in a liquid test sample and methods for preparing and using the immobilized hapten reagent. The immobilized hapten reagent comprises a hapten moiety covalently linked substantially only to the external surface of a polyacrylamide gel particle. The immobilized hapten reagent is prepared by forming a reaction mixture comprising the hapten moiety and the carrier material in a nonswelling solvent wherein the gel particle is substantially impervious to the hapten moiety and wherein an analytically insignificant amount of the hapten moiety is nonspecifically bound to the gel particle. The immobilized hapten reagent is characterized by being substantially stable in aqueous solutions and exhibits an insignificant amount of leakage of the hapten moiety into a liquid test medium.
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申请公布号 |
US4822747(A) |
申请公布日期 |
1989.04.18 |
申请号 |
US19860939902 |
申请日期 |
1986.12.09 |
申请人 |
MILES INC. |
发明人 |
JOHNSON, RICHARD D.;RUNZHEIMER, H. VOLKER;SOMMER, RONALD G.;YIP, KIN F. |
分类号 |
G01N33/53;G01N33/543;G01N33/547;G01N33/548;G01N33/72;G01N33/96;(IPC1-7):G01N33/547 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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