| 摘要 |
PURPOSE:To obtain alpha-N-acetylgalactosaminidase (alpha-GalNAcase) from a culture mixture, by cultivating a fungus belonging to the genus Acremonium, capable of producing alpha-GalNAcase. CONSTITUTION:A fungus such as Acremonium sp. No.413 (FERM P-9585) belonging to the genus Acremonium, capable of producing alpha-GalNAcase is aerobically cultivated (pH6.5 of the medium, 28 deg.C culture temperature and 72hr culture time) and alpha-GalNAcase is separated from supernatant liquid of the culture from which the cell is removed. The enzyme acts on a saccharide chain containing the end bonded to GalNAc by alpha bond to isolate GalNAc and substantially hardly shows activity against p-nitrophenyl-glycoside except p- nitrophenyl-alpha-GalNAc. |
