| 摘要 |
The present invention relates to a kit and a method for detection of an miRNA and, more specifically, to an miRNA detection kit and an miRNA detection method using the kit, the kit comprising: a primer for reverse transcription, which consists of a first hybridization oligonucleotide comprising a nucleic acid sequence which hybridizes specifically to the 3'-end of an miRNA or a poly A tailed miRNA to which poly A is linked via a poly A polymerase, and a first adaptor oligonucleotide comprising any nucleic acid sequence which does not hybridize to a target miRNA; a primer for extension, which consists of a second hybridization oligonucleotide capable of hybridizing specifically to a cDNA reverse transcribed from the miRNA or the poly A tailed miRNA excluding a portion corresponding to the 3'-end of the miRNA or the poly A tailed miRNA hybridized to the first hybridization oligonucleotide, and a second adaptor oligonucleotide comprising any nucleic acid sequence which does not hybridize to the cDNA; and a forward primer selected within the second adaptor oligonucleotide. |
