HUMAN PAPILLOMAVIRUS TYPE DIAGNOSIS WITH NUCLEOTIDE PROBES

摘要

A rapid in situ hybridization assay for detecting the presence of Human Papillomavirus (HPV) in a non-frozen cellular smear fixed on a slide or other support in the absence of aldehyde-based cross-linking reagents. The assay can be conducted in less than four hours and utilizes readibly detectable nucleic acid probes, typically labelled by nick-translation with biotin, for binding to an avidin or streptavidin labelled enzyme, or with an isotope for visual detection.