Protein assay device and method.

摘要

An IgG dipstick or other device is disclosed for the colourimetric detection of IgG in a sample in the presence of other proteins. The dipstick or other device comprises a porous ion exchange member 1, e.g. of ion exchange filter paper, into which the IgG containing sample is drawn by capillary attraction. The pH of the sample is adjusted to the isoelectric point of IgG, and due to the faster diffusion rate of IgG at its isoelectric point as compared with other proteins in the sample which have different isoelectric points, IgG is preferentially separated from the other proteins and its presence is detected by a band of protein reactive dye 2 incorporated into the porous ion exchange member at a predetermined distance from the point of application of the protein sample.