| 摘要 |
PURPOSE: To efficiently adsorb the metalloprotein in whey and to elute and extract the metalloprotein by coating the wall surfaces of inorg. porous particles to be adsorbed with the metalloprotein with the films of aminated polysaccharides having acidic functional groups on the surfaces. CONSTITUTION: At the time of extracting the metalloprotein and more particularly protein bonded with iron (lactophene, transferrin, lactoperoxidase, etc.,) in the whey, the whey is brought into contact with the inorg. porous particles of which the wall surfaces are coated with the films of the aminated polysaccharides having the acidic functional groups on the surfaces. As a result, the metalloprotein is adsorbed on the surface of the porous particles. The metalloprotein is thereafter eluted by an eluent. The polysaccharides are preferably dextran and more particularly preferably diethylaminoethyl dextran. The inorg. porous particles are preferably spherical silica having a diameter of 10 to 1000μm and have pores of an average diameter of 5 to 200nm. |
