PLASMID VECTOR CONTAINING STREPTOMYCIN-SENSITIVE GENE

摘要

PURPOSE:To obtain a DNA, having a nonsense code in a coding region and capable of expressing streptomycin sensitive gene. CONSTITUTION:It is found that a recombinant molecule is a directly selective streptomycin-sensitive plasmid and expression of the sensitive gene is capable of completing a vector (designated as pHSG673 and pHSG683) which can be switched on and off by culture medium condition and species of a host microorganism and has many cloning sites and hardly forming nonrecombinant molecules as the background. One specific example of the DNA has an inductive promoter capable of regulating the expression on the upstream side of the streptomycin-sensitive gene.