Recycle chromatograph with supercritical fluid.

摘要

It is an object to provide a recycle chromatograph with supercritical fluid mobile phase for separating a sample into components by recycling a mobile phase which includes modifier solvent for the purpose of taking a desired component. It is structured in such a manner that a recycling pump (10), a column (14), a detector (16), and a three-way valve (18) are connected in a sequential order as described above to form a closed loop which acts as a recycle flow line. A back-pressure regulator (20) is connected to the remainder port (C) of this three-way valve (18), and a mobile phase supplying device (30, 38, 30A, 38A, 48) for supplying the mobile phase under a predetermined pressure is connected to a flow line between the three-way valve (18) and the recycling pump (10). By means of this mobile phase supplying device and the recycle closed loop (the column pressure drop DELTA p)/(column plate number) can be reduced, whereupon the column effective plate number can be increased. Thus, a sufficient resolution rate for fractionating a component can be obtained after appropriate recycling times. Furthermore, by combining the mobile phase supplying device (30, 38, 30A, 38A, 48) and the back-pressure regulator (20), the internal pressures in the column and the detector can be kept above the critical pressure even if unnecessary components are being removed or a necessary component is being fractionated. As a result of which fractionation can be performed.