| 摘要 |
PURPOSE:To obtain a plasmid vector useful for breeding of arboreous plants, by inserting a chloroplast DNA fragment of back pine containing subunit gene of RuBisCo and H<+>-ATPase into a plasmid derived from Escherichia coli. CONSTITUTION:Chloroplast DNA of black pine is cleaved with a restriction enzyme PstI to provide a Pst-4 fragment (13.7kbp) containing a large subunit of RuBisCo and subunit gene of H<+>-ATPase. The resultant fragment is then inserted into a plasmid derived from Escherichia coli. Thereby the aimed plasmid vector is obtained. |
