| 摘要 |
<p>Recombinant constructs of DNA are provided which are useful for the expression of a heterologous polypeptide in a bacterial host. These constructs contain a novel promoter, the r3 promoter. Variations on the basic construct are made which enhance the level of expression directed by the r3 promoter. These variations are the inclusion of sequences containing strong ribosomal binding sites, and containing A-T rich sequences. In addition, the expression directed by the r3 promoter may be controlled by the insertion of an operator sequence into the construct.</p> |
