Method for obtaining a papain-free antibody fragment preparation.

摘要

A method for obtaining a papain-free F(ab min )2 antibody fragment preparation derived from papain digested whole IgG antibody employing an antibody to papain for binding to and removing papain-associated digestion products from the preparation. The method is particularly useful for digesting whole IgG antibodies which are otherwise sensitive to pepsin digestion to provide F(ab min )2 antibody fragments from which Fab min antibody fragments can be obtained having available free sulfhydryl groups in the hinge region.