| 摘要 |
PURPOSE:To make it possible to measure a minute amount of sample liquid and to make biosensor compact, by using a space between the surface of a substrate, on which physiological active material is immobilized, and the surface of a substrate, on which a chemical-material sensitive transducer is provided, with respect to a pair of the substrates as a sample adding space. CONSTITUTION:In a batch type glucose sensor, a chemical-material sensitive transducer is provided on a facing surface 10a of a glass substrate 13. The surface 10a faces a glass substrate 13. The glass substrate 10 is supported with a holder 17 in parallel with the substrate 13, with a minute interval being provided. A measuring anode 11 and a cathode 12 are formed on the facing surface 10a as thin gold film electrodes. A physiological-active-material immobilized film 14 is laminated on a facing surface 13a of the substrate 13. The surface 13a faces the substrate 10. The film 14 is a mixed LB film of stearyl trimethylammonium and methyl arachidate, in which glucose oxidase is adsorbed. The substrate 13 is also supported with the holder 17. In this way, the substrates 10 and 13 are arranged to that the electrodes and an enzyme immobilized film face each other. The interval of the space formed between the substrates is about 1mm. Said space becomes a sample chamber 15. |
