| 摘要 |
PURPOSE:To efficiently obtain the titled enzyme useful for producing a blended amino acid substance, etc., by cultivating a specific bacterium belonging to the genus Pseudomonas or Trichosporon in a nutrient culture medium. CONSTITUTION:Pseudomonas.fluorescens IFO12055 or Trichosporon sp. 107-1 (FERM P-9489) is cultivated in a culture medium containing 0.5-2% (wt./vol.) carbon source, 0.3-1% (wt./vol.) nitrogen source, 0.05-0.1% (wt./vol.) magnesium, 0.001-0.003% (wt./vol.) calcium, manganese and ferric ion and 0.5-1% (wt./vol.) glycyl-L-proline, polypeptone, etc., at pH5-8 and 20-40 deg.C for 18-40hr. Microbial cells are then collected from the resultant culture and crushed to isolate the aimed proline dipeptidase from the obtained crude extract solution. |
