Methods and probes for detecting nucleic acids.

摘要

An in solution hybridization method utilizes two soluble oligonucletide probes. One of the probes has a homopolynucleotide tail which is captured by a solid support coated with a complementary homopolynucleotide. A ribosomal RNA sequence specific to M. pneumoniae and synthetic probes complementary thereto are provided. Methods are provided for specifically identifying the presence of Mycoplasma pneumoniae 16S ribosomal RNA in a sample by using at least one synthetic nucleic acid probe complementary to the 16S ribosomal RNA in a hybridization assay. The methods comprise providing at least one synthetic probe which has a sequence complementary to at least a portion of a ribosomal RNA sequence specific to M. pneumoniae.