PROCEDEU DE OBTINERE A SERULUI ANTI ALFA 1-GLICO-PROTEINA ACIDA UMANA

摘要

Human anti-alpha-glyco-protein acid serum is obtd. by concentrating human urine contg. approx. 30 mg/100 mls. alpha-glyco-protein, to at least 1/20 of its original vol. This is centrifuged at 6-7000 rpm for 30 mins. followed by the addn. of ammonicerusulphate until a concn. of 30g/100 mls. is reached. The soln. is allowed to stand at room temp. for 24 hours. Centrifuging is repeated now at 10000 rpm for 30 mins. The supernatant is concentrated to 1/40th of the vol. of the previously conc. urine, and passed through chromatographic column of A- SEPHAROSE 4B. The column is eluted with an acetate buffer. Finally large animals are immunised with the prepn. and the immune serum is collected.