AMPEROMETRISK FREMGANGSMAADE TIL KVANTITATIV BESTEMMELSE AF 1,4-DIHYDRONIKOTINAMID-ADENIN-DINUKLEOTID (NADH) I OPLOESNING

摘要

A process for quantitatively determining 1,4- dihydronicotinamide adenine dinucleotide (NADH) in solution is described. The process comprises bringing the NADH-containing solution into contact with an activated carbon electrode, holding the carbon electrode at a controlled, predetermined potential which is able to bring about oxidation of the NADH at the electrode surface, and measuring the current emission from the carbon electrode, with use being made of a precious metal- containing, preferably platinized or palladiumized, activated carbon electrode which comprises a porous, heterogeneous, resin-bound layer of activated carbon or graphite particles which comprise the finely divided precious metal, which is preabsorbed on them, and which are bound together by a natural or synthetic resin binder, preferably a hydrophobic resin such as polytetrafluoroethylene.