| 摘要 |
<p>A method of producing phosphatidylglycerol (PG) by enzymatic conversion of phosphatidylcholine (PC) in the presence of glycerol. The reaction is carried out in a single-phase reaction system containing phosphatidylcholine (PC), phospholipase D enzyme, glycerol, and a soluble calcium salt in an aqueous solvent containing between about 5 %-25 % of a water-miscible alkyl diether, such as 1,1-dimethoxymethane, 1,2-dimethoxyethane, 1,2-dimethoxypropane, and 1,1-diethoxymethane. The reaction goes through a PG maximum which determines the optimum amount of enzyme added to the mixture. After termination of the reaction, the insoluble calcium salts in the mixture, including the calcium salt of PG, are removed and extracted to obtain purified PG, and/or the reaction mixture, which contains phosphatidic acid (PA), is treated further with glycidol, in a suitable alkylgbher, to covert PA to PG. Also disclosed 656a method for converting PA to PG.</p> |
