摘要 |
<p>An assay for 1,25-dihydroxy vitamin D (3) is disclosed. One aspect of the invention involves adding pig receptor (1) protein, radiolabeled 1,25-dihydroxy vitamin D (2) and biotinylated (5) antibody (4) capable of binding to the receptor to untreated blood serum. In performing a competitive binding assay, vitamin D transport protein (7) acts as a screen to minimize interference from related metabolites (8). A kit and an assay are disclosed.</p> |