| 摘要 |
Novel methods useful in the analysis of antigen/antibody reactions and the study of antigenic determinants and immunoglobulins are provided. A novel method is provided wherein an immortal cell line capable of expressing an immunoglobulin molecule, which molecule is the anti-idiotype of an antigen binding substance of interest, is substituted for the antigenic determinant or epitope bound by the antigen binding substance. The cells of the cell line are manipulated to provide the immunoglobulin as a cell surface marker. This results in a stable, efficient source of an antigenic determinant of interest, makes accessible previously unaccessible epitopes and amplifies markers previously found on substances not amenable to FACS analysis. |
