Phosphorylating reagents and method of using the same.

摘要

Novel phosphorylating reagents are provided which are useful in DNA synthesis and purification procedures. The reagents are substituted phosphines which are particularly useful in the phosphorylation of nucleoside and solid supported oligonucleotides at the 5 min -hydroxyl position. Phosphorylation using these reagents is easily and accurately monitored colorimetrically.