| 摘要 |
PURPOSE:To clone the heterologous gene factor to various other microbial strains at the same time using a vector comprising the plasmid of a novel Bacillus stearothermophilus having a tetracycline-resistance gene, a molecular weight of about 2.8 M-Dalton and containing a plasmid represented by a specific restriction enzyme map. CONSTITUTION:Soil is cultured in a TYS medium under shaking, and subjected to the plate culture on a TYS agar medium containing tetracycline to obtain the colony of a novel Bacillus steraothermophilus 15 strain (FERM-P No.6661). The strain contains a plasmid having the tetracycline-resistance gean in the cell, a molecular weight of about 2.8 M-Dalton and a restriction enzyme map of figure. A heterologous gene can be cloned to Bacillus subtilis and a thermophillic strain at the same time by using the plasmid as a vector. |
