Peptide-beta-lactamase conjugates for enzyme-linked immunoassays

摘要

A reagent for an ELISA determination of an antibody, the reagent comprising a peptide covalently linked to beta-lactamase. The reagent can be used in the following method to detect antibodies in a sample which involves a. contacting the sample with protein A linked to a solid support, b. incubating the sample-protein A linked to the solid support, c. washing the incubated sample-protein A linked to the solid support, d. contacting the washed sample-protein A with the reagent, e. incubating the sample-protein A and reagent, f. washing the incubated sample-protein A-reagent, and g. determining the enzymatic activity of the resultant mass.