PHOSPHINOSLICIN RESISTANT GENE

摘要

Selection of Streptomyces viridochromogenes DSM 4112 using phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistant selectants. The DNA fragment which harbours the phosphinothricin (PTC)-resistance gene is obtained from the complete DNA of these selectants by cutting with NcoI, cloning of a 2.7 kb fragment and selection for PTT resistance. This fragment is suitable for producing PTC-resistant plants, and as resistance marker.