Solid phase enzyme immunoassay test apparatus and process for detecting antibodies.

摘要

A test apparatus and process for its use to simultaneously detect the presence of a variety of specific antibodies in a clinical specimen. The procedure involves an enzyme-linked dot blot immunoassay where an array of specific antigens that are used to detect specific antibodies are separately immobilized on a porous membrane (14) which is itself immobilized on a rigid support (11) to provide a dip stick (10). In the process specific antibodies are detected by sequential incubations of the antigen dip stick (10) in three different solutions of immunochemical reagents. The presence of specific antibody is evidenced by the appearance of a blue-violet color change to a dot of a specific antibody immobilized on the porous membrane (14) while the absence of color developement indicates the absence of such specific antibody.